Fig. 9. The effect of Ca²⁺ modulators on GSK-3 phosphorylation. The cells were allowed to spread on fibronectin for 120 minutes and then treated with 50 nM of staurosporine alone (G,H) or together with thapsigargin (100 nM; A,B), PLC-1 inhibitor U-71322 (4 µM; C,D) or BAPTA-AM (10 µM; E,F) for either 0-60 minutes or left untreated for 60 minutes (C60'). The cell lysates were separated by SDS-PAGE and immunoblotted with antibodies recognizing GSK-3/ß phosphorylated on serine 21/9 (A,C,E,G) or total GSK-3 (B,D,F,H).