Fig. 5. The role of GSK-3 in E-lam formation. To investigate the role of GSK-3 in E-lam formation, the cells were treated with LiCl₂ (2-40 mM; A) or SB-415286 (1-50 µM; B) in presence or in absence of staurosporine. The percentage of cells that were spread or representing E-lams was calculated. Results (mean±s.d., n=8) of one representative experiment are shown. (^*P<0.05; ^**P<0.01; ^***P<0.001.) (C) The morphology of cells treated with 50 nM staurosporine only (a) or in presence of 30 µM SB-415286 (b). E-lams are marked with arrows. Scale bar: 10 µm.