Fig. 3. R-Ras in focal adhesions. Cells were transfected with pEGFP-R-Ras38V (R-Ras38V), and pEGFP-R-Ras43N (R-Ras43N) fixed, stained for vinculin and analysed by confocal microscopy. Note that R-Ras43N does not colocalize with vinculin, whereas R-Ras38V shows a strong colocalization. In R-Ras-38V/Rac1-12V the pCDNA4-TO-R-Ras38V vector was cotransfected with pEGFP-Rac12V and stained with an antibody against R-Ras. Arrowheads indicate colocalization of R-Ras and EGFP-Rac1 to focal adhesions. Bars, 10 µm.