Fig. 2. Identification of proteins interacting with vFLIP in a KSHV-infected B-cell line. Proteins were immunoprecipitated from the KSHV-infected PEL cell line BC3 using a rat IgG control or the anti-vFLIP monoclonal antibody 6/14 and then run on a 12% SDS-polyacrylamide gel. (A) Silver stain of tracks containing 10% of the immunoprecipitated protein. (B) Colloidal Coomassie stain of part of a parallel pair of tracks containing 90% of the immunoprecipitated protein. Bands detected in the vFLIP immunoprecipitate, but not the control, were excised from the Coomassie stained gel and identified by mass spectrometry as indicated.