Fig. 6. The CD2-RB3 tubulin-sequestering chimera blocks MAP4-mediated MT stabilization. Cotransfected K562 cell lines (DNA ratio 1:1 of MAP4/Vector-Co:CD2-chimera/Vector-Co) were induced with Cd²⁺ for 20 hours. Total cellular lysates (20 µg/lane) were separated by 12% SDS-PAGE and immunoblots were probed with the antibodies indicated. Mean MAP4-specific fluorescence intensities, as determined by flow cytometry, are given below the autoradiograph. Mean MT-specific fluorescence within the G2 populations was determined after 20 hours of induced expression and is shown in the bottom panel. All data in this figure are derived from the transfected cell populations analyzed in Fig. 5 but are representative of at least three independent transfection experiments.