Fig. 4 (A,B) Membrane redistribution of junctional proteins in MCF-7B cells upon long-term absence of estrogen. Coverslip-grown confluent cultures were transferred into estrogen-deprived medium for 16 days. IF localization of Bcl-2 and various junctional proteins was carried out as outlined in the legend of Fig. 2. Double staining was performed by detecting two different primary antibodies with FITC- or rhodamine-conjugated species-specific secondary antibodies. Merged images (right column, A: bottom row, B) were constructed using the Adobe Photoshop software. CK, cytokeratins; Pg, plakoglobin. Arrows in A indicate the absence of Bcl-2 expression or membrane localization of junctional proteins. Scale bars: (A) 150 µm; (B) 75 µm