Fig. 3. (A) Deficient production of KGF and GM-CSF in fibroblasts co-cultured with HaCaT cells. Kinetics of KGF and GM-CSF production, measured in culture supernatants of co-cultures with NEK and HaCaT cells, of 2-day-conditioned media, by ELISA and calculated as pg/10⁵ cells. Bars represent means ± s.d. of duplicate measurements performed in three independent experiments. (B) Ineffectiveness of KGF, GM-CSF or IL-1 on epidermal tissue restoration by HaCaT cells in organotypic co-cultures with postmitotic fibroblasts. Organotypic co-cultures of HaCaT cells with postmitotic fibroblasts (3x10⁵/ml) in collagen type-I gel were grown for 10 days in DMEM with 10% FCS (Control) and after addition of IL-1 (5 ng/ml), KGF (10 ng/ml) or GM-CSF (100 ng/ml); medium was changed every second day (H and E staining; bar, 100 µm).