Fig. 1. Activation and nuclear accumulation of Dd-STATc by osmotic stress. Ax2 cells were allowed to develop for 4 hours in shaken suspension, were harvested and then divided into two. One portion was incubated in KK2 containing 200 mM sorbitol and the other portion was incubated in KK2 containing 100 nM DIF. At the indicated times, one aliquot was harvested and the specific tyrosine phosphorylation level of Dd-STATc was determined by western transfer (A). At the same time, a separate aliquot was analysed immunohistochemically to determine the proportion of cells showing nuclear enrichment of Dd-STATc (B). The results are shown as the mean±s.d. but the error bars at 5, 10 and 15 minutes for the sorbitol treated sample are so small as to be obscured by the filled boxes.