Fig. 2. AIP1 but not SETA co-immunoprecipitates with cytoskeletal proteins. SETA or Flag-tagged AIP1 were transiently transfected into HEK293 cells and various cytoskeletal proteins were immunoprecipitated with specific antibodies, as indicated. The immunoprecipitates were subsequently analyzed by PAGE and immunoblotting with polyclonal anti-SETA antibody or monoclonal anti-Flag antibody. Although SETA was present in the lysates of the cells (lane 11), and was immunoprecipitated with the positive control protein EGFR (lane 10), it was not found in any of the immunoprecipitates made with antibodies to cytoskeletal proteins (lanes 1 to 9). In contrast, AIP1 revealed a strong signal after immunoprecipitation of most cytoskeletal proteins, with actin, -tubulin, ß-tubulin and phosphotyrosine tubulin, as well as the microtubule-associated proteins (MAPs), showing particularly strong signals, and was also found in EGFR immunoprecipitates.