Fig. 1. SETA co-localizes with microfilaments and microtubules. Confocal microscopy of astrocytes stained with polyclonal anti-SETA antibody together with FITC-conjugated secondary antibodies (A,D,G,J), TRITC-phalloidin (B,E), or ß-tubulin followed by TRITC-conjugated secondary antibodies (H,K). The analysis revealed partial co-localization of SETA and actin microfilaments, and focal adhesions (arrows in A-C). However, areas without co-localization were also detected (arrowheads in D-F). Furthermore, partial overlap of SETA and ß-tubulin staining was detected in the peri-nuclear region and along individual bundles of microtubules (arrows in G-L). As shown for actin, the overlap was not complete (arrowheads in J-L).