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Fig. 4. Endogenous archvillin mRNA is clustered at the ends of myotubes, but no clustering is observed for supervillin mRNA in polarized human fibroblasts or for archvillin mRNA in myoblasts. (A) WI-38 fibroblasts, (B) 50MB-1 myoblasts and (C,D) 50MB-1 myotubes were fixed, probed with digoxigenin-labeled sequences conserved in human supervillin and archvillin mRNAs, and visualized with rhodamine-labeled anti-digoxigenin. Note the strong granular staining at the tips of myotubes (arrows); the most proximal of the centrally localized nuclei is indicated (nucleus). Bars, 5 µm.