Fig. 7. Hypoxia increases ROS production in renal cell carcinoma. Caki-1 cells were incubated with 10 µM DCFH in hypoxic conditions for 0 to 240 minutes. Production of ROS was measured by fluorescence emitted from oxidized DCF. After hypoxia, cells were lyzed and fluorescence was measured at 530 nm (A). To inhibit ROS production, cells were preincubated with 10 uM DPI then exposed to hypoxic or normoxic conditions for 1 hour (B). Fluorescence data are represented relative to normoxic values. Means ± s.e.m. were calculated from three independent experiments. Significant differences (P<0.1) are indicated by asterisks (*).