Fig. 8. RVXF peptides lead to apoptosis when introduced into G1-phase nuclei. (A) RAXF or RVXF peptides were transfected into G1-(left six panels) or S-phase (right four panels) cells as in Fig. 7 and cells were cultured for 12 hours. Cells were analyzed by TUNEL and DNA labeling using Hoechst 33342. Bars, 20 µm. (B) Proportions (±s.d.) of TUNEL-positive cells treated as indicated in G1 or S phase (n=300 cells/treatment in duplicate experiments).