JCS -- Solan et al. 116 (11): 2203 Figure IG1

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 1. The p368 antibody reacts with Cx43 only when S368 is present. Shown is an immunoblot of whole cell lysates from HeLa cells transfected with wild-type (wt) Cx43 or Cx43 containing a S368A mutation. Cells were either incubated in the presence (TPA) or absence (CON) of 50 ng/ml TPA for 30 minutes. The immunoblot was probed with either an antibody to the N-terminal region of Cx43 ( ${alpha}$ -Cx43) or the anti-p368 antibody ( ${alpha}$ -p368). Positions of the molecular weight markers are shown on the left.