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Fig. 4. Chromatin dynamics in guard-cell and pavement-cell nuclei. Overall mean-square differences in distance between GFP spots <{Delta}d2> plotted against elapsed time intervals <{Delta}t> in guard-cell ({square}) and pavement-cell ({blacksquare}) nuclei of homozygous EL702C seedlings. Each data point represents an average of data collected from at least nine independent experiments. Open circles ({circ}, guard-cell nuclei) and closed circles (•, pavement-cell nuclei) represent data from fixed seedlings, which were imaged under identical conditions with data points representing averaged values from at least six independent experiments and nine nuclear observations. Vertical bars at each time point show the standard errors for the particular set of data collected. If the motion were not restricted, <{Delta}d2> would continue to increase with increasing time. The longer the time interval between measurements, the greater the distance between two diffusion points is expected to change.