Fig. 3. Nuclear properties of guard cells and pavement cells in homozygous EL702C seedlings. (a) GFP spot numbers observed in guard-cell nuclei (open column) and in pavement-cell nuclei (closed column). Spot numbers were counted in a total of nine guard-cell nuclei and ten pavement-cell nuclei whose chromatin movements were measured. The mean spot numbers per nucleus are shown with the standard deviations from the mean indicated. The number of samples analysed is indicated by `n' on the graph. (b) Relative nuclear DNA contents of guard-cell nuclei (open column) and pavement-cell nuclei (closed column). The samples were stained with DAPI and the nuclear intensities in guard cells (total of 17 in three seedlings) and pavement cells (total of 15 in three seedlings) were compared with that in root-tip cells (whose ploidy is known to be 2C). The mean relative intensities are shown with the standard deviations from the mean. The number of samples analysed is indicated by `n' on the graph. (c) Nuclear volumes in guard-cell nuclei (open column) and pavement-cell nuclei (closed column). The nuclear volumes in a total of nine guard-cell nuclei and ten pavement-cell nuclei whose chromatin movements were measured were calculated by measuring the non-lac-operator-binding GFP signals in nuclei. The mean volumes per nucleus are shown with the standard deviations from the mean indicated. The number of samples analysed is indicated by `n' on the graph.