Fig. 2. The distribution of the dihydropyridine receptor (L-type Ca²⁺ channel) and the perlecan core protein are unaffected in NDST-1^–/– skeletal muscle. Immunofluorescence staining for (a,b) HS-epitope AO4B05, (c,d) DHPR, and (e,f) perlecan core protein in (a,c,e) wild type and (b,d,f) NDST-1^–/– skeletal muscle. Overall staining for HS epitope AO4B05 is significantly reduced in (b) NDST-1^–/–, compared to (a) wild-type muscle, whereas staining (c,d) for DHPR and (e,f) for perlecan remains virtually unaltered. Note the persistence of staining in neural tissue in (b) (n=3). Bar, 75 µm.