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Fig. 4. Localization of laminin in SEY (+/–) mouse corneas. Laminin was detected by immunofluorescence in the basement membrane underlying the corneal epithelium and the endothelium of wild-type (A) and mutant (+/–) Pax6SeyDey (B) corneas. The dim fluorescence present in wild-type epithelial cells was absent in mutant cells, and large aggregates of immunofluorescence (asterisks in B) occasionally appeared in the stroma of mutant corneas. Similar staining was observed in the Pax6SeyNeu (+/–) cornea (data not shown). Abbreviations: c, epithelium; s, stroma; n, endothelium.