Fig. 6. Detection of CD55 on wild-type eggs by western blotting. Eggs, PI-PLC-treated, +, (right lane) or untreated, –, (center lane), were solubilized and separated by reducing SDS-PAGE. PVDF membranes of transferred proteins were probed for CD55. The left lane is staining from a positive control lysate of mouse CTLL-2 cells, a line known to express CD55. The slightly larger size of the CD55 in the CTLL-2 cultured cells may reflect a difference in CD55 glycosylation.