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Fig. 2. Surface expression of eGFP-GPI on wild-type or Pig-a–/– oocytes. Oocytes from wild-type and conditional knockout females carrying the eGFP-GPI transgene were stained with anti-GFP to determine the level of eGFP expressed on the surface as a GPI-AP. (A) Transmission and fluorescence images of wild-type oocytes. (B) Transmission and fluorescence images of Pig-a–/– oocytes.