Fig. 2. Surface expression of eGFP-GPI on wild-type or
Pig-a/ oocytes. Oocytes from wild-type and
conditional knockout females carrying the eGFP-GPI transgene were stained with
anti-GFP to determine the level of eGFP expressed on the surface as a GPI-AP.
(A) Transmission and fluorescence images of wild-type oocytes. (B)
Transmission and fluorescence images of Pig-a/
oocytes.