Fig. 8. Dominant-inhibitory dynamin2 K44A inhibits LPA₁ internalization. (A) LPA₁-expressing HeLa cells were incubated for 30 minutes in the absence (Untreated) or presence of 10 µM LPA prior to indirect immunofluorescence localization of LPA₁. (B) Stable LPA₁ transfectants were transiently transfected with plasmids encoding either WT Dyn2-GFP or dominant-inhibitory Dyn2-GFP K44A. The cells were then incubated with 10 µM LPA for 30 minutes, fixed and processed for indirect immunofluorescence localization of LPA₁ using mouse anti-FLAG antibodies followed by Alexa594-labeled goat anti-mouse IgG. Dynamin localization was determined by direct visualization of GFP fluorescence. Bar, 10 µm.