Fig. 5. Agonist removal stimulates the recycling of internalized LPA1
back to the PM. (A) Stably transfected HeLa cells expressing LPA1
were incubated in the absence (Untreated) or presence (+LPA) of 10 µM LPA
for 30 minutes. (B) The cells were then rinsed, incubated in serum-free medium
for the indicated times and processed for indirect immunofluorescence
localization of LPA1. Bar, 10 µm.