JCS -- Carnegie et al. 116 (10): 1905 Figure IG2

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Fig. 2. Co-elution of PPP4c and Flag-R2 from an anti-Flag agarose column. HEK 293 cell lysates were incubated with anti-Flag agarose as described in Materials and Methods. Fractions (12x1 ml) eluted with Flag peptide from the anti-Flag agarose column were collected and analysed by SDS-PAGE and subsequent immunoblotting with anti-Flag (for detection of Flag-R2) and anti-PPP4c antibodies.