Fig. 4. Subcellular localization of DsRed-EB1, DsRed-EB1CT and DsRedEB1NT in MDCK cells. (a-f) Basal sections of cells expressing full-length DsRed-EB1 (a-c) or DsRed-EB1CT (d-f) and co-stained with a mouse monoclonal antibody to ß-tubulin (green). (a-c) DsRed-EB1 predominantly localizes to the distal (plus) ends of MTs (arrows in a-c). (d-f) DsRed-EB1CT is enriched in cortical clusters at the tip of the cell extension (arrowheads in d-f) and occasionally localizes to the plus end of MTs (arrows in d-f). (g-i) Basal sections of cell extensions of a MDCK cell expressing DsRed-EB1NT (red, marked with `+') and an untransfected MDCK cell marked as `-'. Cells were costained with a mouse monoclonal antibody to EB1 (green) that does not recognize DsRed-EB1NT (see Material and Methods). DsRed-EB1NT co-aligns along MTs including their distal (plus) ends, which are marked by endogenous EB1 (green) but is not as well restricted to the distal (plus) ends of MTs as endogenous EB1 (arrows in g-i). Bar, 10 µm.