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Fig. 2. Analysis of licensing factors upon re-entry into cell cycle. NHDF cells were arrested in G0 and stimulated with 10% serum to enter cell cycle. Samples were taken at the indicated times after serum stimulation. (A) DNA content was measured by FACS. (B) Total cell extracts were prepared and hCdt1, geminin, Cdc6 and cyclin A protein levels were analyzed by western blot.