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Fig. 6. {alpha}-actinin changes the organization of both the F-actin and the cytokeratin network. Low magnification (left panels) and high magnification (right panels) double-label confocal micrographs showing F-actin (red) and cytokeratin (green) in rapidly frozen extract samples prepared after the addition of {alpha}-actinin. The exogenous {alpha}-actinin results in fine, highly crosslinked meshworks of F-actin cables in extracts that colocalize with cytokeratin. At high magnification, cytokeratin localization along F-actin cables in these networks appears punctate. The bar graph shows that the addition of exogenous {alpha}-actinin significantly decreased the mean distance between both adjacent F-actin cables and cytokeratin relative to control samples (P<0.05).