Fig. 1. Levels of PACE4, PC7 and furin transcripts in 3T3-L1 cells during adipocyte conversion. (A) Total RNA extracted from 3T3-L1 preadipocytes and adipocytes was analyzed by semi-quantitative RT-PCR as described in Materials and Methods. RNA from epididymal WAT was also examined for comparison. (B) Ratios between PC and L30 densitometric values obtained by semi-quantitative RT-PCR were determined. They are presented as percent of maximal expression of each PC. The values represent means±s.e.m. of five independent experiments.