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Fig. 4. Integrin activation caused Src-dependent SHIP2 phosphorylation in other cell types. Anti-SHIP2 or control preimmune (Pre) IPs were blotted with anti-phosphotyrosine ({alpha}-PY) or anti-SHIP2 antibodies. Samples were from cells that were adherent (Ad), detached (D) or freshly re-plated (RP) on collagen-I-coated dishes (6 µg/cm2) in the presence of DMSO (-) or 1 µM Src inhibitor compound PD173956 (+) for 60 minutes. The arrows point to tyrosine-phosphorylated SHIP2. (A) SH-SY5Y neuroblastoma cells. (B) MDCK cells.