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Fig. 1. Collagen I induces SHIP2 tyrosine phosphorylation. Anti-SHIP2 or control pre-immune (Pre) immunoprecipitates (IP) from HeLa cells were blotted with anti-phosphotyrosine ({alpha}-PY). Anti-SHIP2 blots show amounts of SHIP2 protein in respective IP samples. The arrows point to tyrosine-phosphorylated SHIP2. (A) Time course: adherent (Ad), detached (D) or cells that were freshly plated on a collagen-I-coated (6 µg/cm2) surface (C.I) at indicated post-plating intervals were used for IP. (B) Coating density dependency: IPs were carried out as above. Cells were re-plated for 60 minutes on a polystyrene surface coated with increasing concentrations of collagen I (0.1 µg/cm2 to 6 µg/cm2).