Fig. 2. Neuronal transduction using EIAV. (A,B) Brightfield (A) and fluorescent (B) image of dissociated dorsal root ganglia transduced with pONY8.0G. At a multiplicity of infection (MOI) of 10, 45% of cells in this culture were transduced as shown by GFP fluorescence in B. (C-E) Confocal microscopy images of the same two cells in primary cultures of striatal neurons transduced with the EIAV construct, pONY8RARß2.Flag. In C a RARß antibody and in D a Flag antibody were used to visualize transduced cells with Texas-Red- and FITC-coupled secondary antibodies, respectively. In E, the images are merged to show the colocalization (yellow) of the RARß and Flag antibody staining.