Fig. 4. Slt2p phosphorylation in wild-type and rho5 cells under inducing conditions at 37°C. 50 µg of total protein was loaded in each lane and immunological detection was performed as described previously (Lorberg et al., 2001). Phosphospecific antibodies were used to detect the dually phosphorylated kinase in the upper lane. In the lower lane a polyclonal antiserum was employed as a loading control.