Fig. 7. Local expression of PtdIns4P 5-kinase (PI4P5K) and local activation of ERM proteins. HA-tagged PtdIns4P 5-kinase was overexpressed in serum-starved A431 cells that were triple stained with anti-HA mAb (A), anti-ERM pAb, TK89 (B) and anti-CPERM mAb (C). PtdIns4P 5-kinase was occasionally concentrated on the surface of vesicular structures (A) where ERM proteins were recruited (B) and phosphorylated (C). Bar, 10 µm.