Fig. 4. Enhanced vascular permeability in K14-PlGF mice. (A,B) Evans blue dye-perfused transgenic mice show increased extravasated blue tracer in the skin compared with perfused control mice. (C,D) Whole mount ear preparations from 10-week-old mice perfused with biotinylated R. communis lectin. Numerous leakage sites are present in the ear vessels of transgenic mice (D, arrows). Bars, 100 µm. (E) Miles vascular permeability assay. 1-4: 25, 50, 125, 250 ng of injected PlGF, respectively; 5: site of PBS injection.