Fig. 2. Hypervascularized skin phenotype of PlGF-transgenic mice. (A) Newborn mice are visibly redder than wild-type controls. (B) The redder color of the skin in transgenic mice persists in the adult (10-week-old). Enlarged vessels are evident in the ear of transgenic mice compared with controls. (C,D) Whole mount ear preparations from control (C) and transgenic (D) 10-week-old mice perfused with biotinylated-L. esculentum lectin showing the increase in vessel number, tortuosity and size in the transgenic ears. (E,F) Signs of active angiogenesis in transgenic mice: vessel spike emission (E, arrowheads), intussusception (E, arrows) and glomeruloid bodies (F). Bars, 100 µm.