Fig. 1. PlGF overexpression in the skin of transgenic mice. In situ hybridization analysis with an antisense PlGF riboprobe on the back skin of wild-type (A,B) and transgenic (C,D) mice. (A,C) Bright field; (B,D) dark field. Bars, 100 µm. (E) RNase protection assay for PlGF on total RNA from back skin biopsies of wild-type (WT) and transgenic (TG) littermates. The murine melanoma cell line M3 was used as a positive control for the endogenous PlGF transcript (E). (F) Western blot for PlGF on skin lysates. As a control, mouse recombinant PlGF (mrPlGF) was included. (G) ELISA for PlGF on 24-hour-conditioned medium of cultured keratinocytes from newborn transgenic and wild-type mice.