Fig. 3. Immunofluorescence images of cytokinesis in polyspermic zygotes of S. lomentaria. (A,C,E,G) DIC images; (B,D,F,H) merged images of immunolocalization of ß-tubulin (green), centrin (red) and DAPI staining (blue). (A,B) Anaphase of a multipolar spindle. Four daughter nuclei are formed. Arrowheads in B show four anti-centrin-positive spots. (C,D) Four daughter cells are produced. Each of the four daughter cells contains one nucleus and one centrosome. Arrows in C show cytokinetic planes and arrowheads in D show anti-centrin-positive spots. (E,F) Three daughter cells are produced. Each of the three daughter cells contains one nucleus. One of the three cells contains two centrosomes. These centrosomes are close to each other and function as one MTOC. Arrows in E show cytokinetic planes and arrowheads in F show anti-centrin-positive spots. (G,H) Four daughter cells are produced. Each of the four daughter cells contains one centrosome. The top cell has no nucleus. Arrows in G show cytokinetic planes and arrowheads in H show anti-centrin-positive spots.