Fig. 9. Intracellular localization of VSG-hydrazine-biotin. Bloodstream-form trypanosomes were grown for 44 hours in the presence (b) or absence (a) of 0.5 mM hydrazine. Parasites were fixed in HBS buffer containing 1% formaldehyde. VSG was immunolocalized using anti-VSG MITat 1.2 specific antibodies. Bars, 5 µm; arrows and arrowheads denote the nucleus and kinetoplast DNA respectively, which were stained by bisbenzimide.