Fig. 8. Effect of tunicamycin and hydrazine or hydrazine-biotin on VSG formation. Trypanosomes were cultivated in the presence of 10 µg ml^-1 tunicamycin (lanes 2, 3 and 4) and 10 mM hydrazine (lane 4) or 10 mM hydrazine-biotin (lane 3) for 4 hours at 37°C in a CO₂ incubator. Following cultivation, cells were analysed by SDS-PAGE and western blotting using anti-VSG MITat 1.2 specific antibodies (a), anti-CRD specific antibodies (b) or streptavidin-HRP (c). Lane 1 shows the control sample incubated in the absence of both tunicamycin and hydrazine.