Fig. 7. Intracellular localization of TbGpi8 in bloodstream-form trypanosomes. Affinity-purified chicken anti-TbGpi8 antibodies have been used to localize TbGpi8 in intracellular compartments. This labelling was localized specifically around the nucleus and in tubular and vesicular structures throughout the cell. (a-d), trypanosomes were sequentially stained with anti-Gpi8 antibodies and FITC-labelled anti-chicken IgG (a), anti-BiP antibodies and TRITC-labelled anti-rabbit IgG (b) and bisbenzamide (c). (d) shows an overlay of all images. (e) overlay of Gpi8- and BiP-stained cells. Bars, 5 µm.