Fig. 6. Western blot detecting TbGpi8 with specific IgY antibodies. Trypanosomal lysate was separated by SDS-PAGE and analysed for TbGpi8 using affinity-purified chicken anti-TbGpi8 antibodies (lanes 2 and 3) or unspecific IgY antibodies isolated from non-immunized eggs (lanes 4 and 5). Lane 1, molecular weight standards, lanes 2 and 4, lysates equivalent to 1x10⁷ cells per lane; lane 3 and 5, lysates equivalent to 5x10⁶ cells per lane.