JCS -- Kang et al. 115 (12): 2529 Figure IG5

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Fig. 5. Transamidase activity of TbGpi8 and MBP-TbGpi8. (a) Trypanosomal lysate and substrate were incubated at 30°C in the presence ( ${blacktriangleup}$ ) or absence ([UNK]) of recombinant TbGpi8. (b) Recombinant TbGpi8 ( ${blacktriangleup}$ ) or MBP-TbGpi8 ([UNK]) and substrate were incubated at 30°C. (c) Trypanosomal lysates were preincubated with Gpi8-specific antibodies for 1 hour at room temperature before the enzyme assay was performed. ( ${blacktriangleup}$ ) control assay without antibodies; ( ${blacktriangledown}$ ) 60 pg/ml antibody; ( ${square}$ ) 120 µg/ml antibody, ( ${blacksquare}$ ) 240 pg/ml antibody, ([UNK]) substrate alone.