Fig. 2. Induction and purification of MBP-TbGpi8. Cells were induced using 0.3 mM IPTG and lysed by sonification. MBP-TbGpi8 was eluted with elution buffer containing 10 mM maltose and analysed by SDS-PAGE. Lane 1, molecular weight markers; lane 2, lysate from non-induced cells; lane 3, cells induced with 0.3 mM IPTG; lane 4, MBP-TbGpi8 after purification using an amylose column.