Fig. 1. The intracellular localization of UL14 protein in 14/Vero cells (A-K) and 14/HEp-2 cells (L) detected by immunofluorescence (A, mock). UL14 protein at 37°C (B) and after heat shock at 43°C for 30 minutes (C). UL14 protein (D) colocalizes with nucleolin (E) after heat shock (F, merge). UL14 protein (G) co-stained with propidium iodide (H) after heat shock (I, merge). UL14 protein localization after 5 hours treatment with ATP-depletion cocktail (J) and 5 hours of 1 M sorbitol treatment (K). UL14 protein localization in M-phase cells after heat shock, co-stained with propidium iodide (L, merged image) shows localization at the centrosomes. Bars, 10 µm.