Fig. 5. Induced overexpression of ASIP/PAR-3 accelerates TER development after cell plating. (A-C) There is no significant difference in cell growth between cells cultured with or without 10 ng/ml of doxycycline (DC). (D-F) TER development of each cell line was measured at different times after plating on Transwell-Clear^TM filters at a density of 1.5x10⁵ cells/cm². Cells were maintained with or without 10 ng/ml of DC for at least 3 days before plating. Every point is the mean±s.e.m. of three groups of cells on independent filters. P-values were calculated with a two-sided t-test and statistical significance was considered at P<0.05 (D, asterisks). (G) Expressions of the T7-tagged ASIPs and endogenous aPKC at the different times after plating. Cells were maintained with or without 10 ng/ml of DC and 5x10⁴ cells/lane were subjected to western blot analysis with the T7-tag-specific antibody (Omni probe) and anti-aPKC antibody.