Fig. 1. (A) Full length ASIP/PAR-3 (ASIP180k) and its splice variant with a shorter C-termini (ASIP150k). The region recognized by each antibody (C2-3AP, A2-2AP and Yap) is indicated; Yap recognizes the specific C-terminal sequence of ASIP150k. CR, conserved region. (B) Expression of ASIP/PAR-3 in various rat tissues. Total extracts from various rat tissues (lung, glandular stomach, kidney, prostate, ovary, uterus), intestinal epithelial cell scrapings (IEC), and COS1 cells transfected with T7-ASIP150k or T7-ASIP180k as positive controls were subjected to SDS-PAGE (8% polyacrylamide gel) followed by western blot analysis with the affinity-purified anti-ASIP antibodies (C2-3AP, A2-2AP and Yap). C2-3AP recognizes bands of 180 kDa (filled arrowheads; lung, glandular stomach, kidney, prostate, ovary and uterus) and 150/160 kDa (unfilled arrowheads; IEC, kidney and prostate), which are recognized by A2-2AP and Yap, respectively.