Fig. 7. Src inhibitor inhibits S1P-induced cortical localization of cortactin. Serum-starved HUVECs were pretreated with the Src kinase inhibitor, PP2 (5 µM), or Y27632 (7 µM), a Rho kinase inhibitor, for 30 minutes at 37°C; S1P was added for an additional 2 minutes before fixing and staining cells for cortactin (red) and actin (green). Confocal images of stained cells are shown.