Fig. 7. Effects of the N/S and A₂G₂ mutations on accumulation of creatine kinase. Myoblasts that had been cultured for 24 hours were transfected with recombinant pcDNAs for Myc-tagged wild-type MARCKS, N/S and A₂G₂. After incubation for 48 hours, the cells were stained with anti-Myc antibody (FITC) and anti-creatine kinase antibody (PE) followed by FACS analysis, as described in Materials and Methods. The level of creatine kinase (CK) in the cells transfected with pcDNA for the wild-type MARCKS was expressed as 100% and the others were expressed as relative values. Each of the data represent the mean±s.e. of triplicate determinations.