Fig. 4. CaM-EGFP redistribution in relation to kinetochores and microtubules in MDCK. (A) Metaphase MDCK control (no drug). (B) Star-like structures in nocodazole (0.1 µg/ml, 4 hours)-treated MDCK. The control cell shows the calmodulin (green) concentrated at the spindle pole in a ring-like shape, the microtubules (red; overlapping green and red seen as yellow) and the kinetochores (white, false colour) at the metaphase plate (blue). The star-like structure in stage III of mitotic spindle disruption seen with four colours gives the detailed arrangement of the four elements (B, detail shown in inset i): calmodulin as a core with radiating microtubules and the corresponding kinetochores attached to chromosomes. (C) CaM-EGFP redistribution relative to kinetochores induced with nocodazole in stage IV of spindle disruption. The kinetochores (red) are situated in immediate proximity to the CaM-EGFP (green) accumulation and chromosomes (blue). Bar, 10 µm.