Fig. 3. Effect of nocodazole (6 hours, 0.1 µg/ml) on CaM-EGFP localisation in MDCK cells. (A) Stage III of microtubule disruption; (i) star-like structure; (ii) centrosomal remnant; (a₁) centrosomal remnant; (a₂) star-like structure in control experiments with cold treatment (4°C, 15 minutes to 2 hours). The star-like substructure shows the CaM-EGFP concentrated centrally with short microtubules radiating from it and the chromosomal material present at the outermost region. The centrosomal remnants show an alternating pattern of tubulin and CaM-EGFP with no obvious colocalisation, seen as `ring' in projection. (B) Stage IV shows CaM location at kinetochore level in the absence of residual microtubules as seen with immunofluorescence. No centrosomal remnants could be seen (green, CaM-EGFP; red, -tubulin; blue, chromosomes; projection of deconvolved series). Bar, 10 µm.