Fig. 2. Calmodulin distribution in the mitotic spindle in control cells and in mild treatment with nocodazole. (A) Control metaphase HeLa (projection of deconvolved sections). (B) Stage I: MDCK (nocodazole 0.01 µg/ml, 2 hours; one section deconvolved). (C) Stage II: MDCK (nocodazole 0.01 µg/ml, 2 hours; projection of deconvolved section; adjacent panel shows only the CaM-EGFP (green) of the same cell). In the control, as in the first two stages of the mitotic spindle perturbation with depolymerising drug, CaM-EGFP is located at polar level in a ring-like pattern, and in the mitotic spindle along microtubules from pole to kinetochore with a decreasing intensity (green, CaM-EGFP; red, -tubulin; blue, chromosomes). Bar, 10 µm.